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https://elib.belstu.by/handle/123456789/14462| Title: | Engineering of a recombinant fab antibody fragment against cortisol |
| Authors: | Dormeshkin, D. O. Leontiev, V. N. Ivashkevich, I. G. Gilep, A. A. |
| Keywords: | молекулярное клонирование генов кортизол праймеры рекомбинантный белок |
| Issue Date: | 2013 |
| Publisher: | BSTU |
| Citation: | Engineering of a recombinant fab antibody fragment against cortisol / D. O. Dormeshkin [et al.] // Proceedings of BSTU. – Minsk : BSTU. – 2013. – № 3. – P. 215-217. |
| Abstract: | The paper dwells upon the molecular cloning of genes coding the structure of mouse immunoglobulins, which can specifically bind steroid hormone cortisol. For these purposes primers covering all families of genes, which code the structure ofimmunoglobulins G. Molecular-genetic constructions for effective expression of recombinant antibodies in cells of Escherichia coliwere constructed. Heterologous expression of Fab-fragments was performed. Recombinant protein has been isolated in homogenous state. |
| URI: | https://elib.belstu.by/handle/123456789/14462 |
| Appears in Collections: | 2013, № 4 |
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