https://elib.belstu.by/handle/123456789/20522 Mon, 20 Apr 2026 11:25:22 GMT 2026-04-20T11:25:22Z https://elib.belstu.by/handle/123456789/14462 Title: Engineering of a recombinant fab antibody fragment against cortisol Authors: Dormeshkin, D. O.; Leontiev, V. N.; Ivashkevich, I. G.; Gilep, A. A. Abstract: The paper dwells upon the molecular cloning of genes coding the structure of mouse immunoglobulins, which can specifically bind steroid hormone cortisol. For these purposes primers covering all families of genes, which code the structure ofimmunoglobulins G. Molecular-genetic constructions for effective expression of recombinant antibodies in cells of Escherichia coliwere constructed. Heterologous expression of Fab-fragments was performed. Recombinant protein has been isolated in homogenous state. Tue, 01 Jan 2013 00:00:00 GMT https://elib.belstu.by/handle/123456789/14462 2013-01-01T00:00:00Z https://elib.belstu.by/handle/123456789/14459 Title: Special properties of free-floating and immobililized active sludge biotic community Authors: Markevich, R. M.; Grebentchikova, I. A.; Rodenko, A. V.; Vostrova, R. N. Abstract: The biotic community composition of free-floating and fiber-porous carrier immobililized active sludge from Ivatsevichi treatment plant aerotanks, functioning under nitri-denitrification conditions, has been studied. Differences in flock structure, species composition and proportion of main indicative groups of free-floating active sludge organisms for different technology lines have been revealed. About 50 species of organisms have been identified. The ability of the fiber-porous carrier to adsorb the considerable amount of sludge biomass has been shown. Tue, 01 Jan 2013 00:00:00 GMT https://elib.belstu.by/handle/123456789/14459 2013-01-01T00:00:00Z https://elib.belstu.by/handle/123456789/14460 Title: Electronic absorption spectra of st. john's wort extracts Authors: Kovalenko, N. A.; Supichenko, G. N.; Leontiev, V. N.; Stasevich, O. V. Abstract: In this study electronic absorption spectra of John's Wort extracts of different types from the collection of Central Botanical Garden of the Belarusian NAS have been investigated. It has been shown that the character of electronic absorption spectra significantly depends on the nature of herbal material, temperature, polarity of solvents and pH value of medium. The Hypericum perforatumwas found to be the most prospective home herbal material. The optimal conditions of hypericin extraction from John's Wort, including temperature mode and pH value have been established. On the basis of spectral researches it is shown that the greatest extent of hypericin extraction is reached at ethanol and acetone use. Tue, 01 Jan 2013 00:00:00 GMT https://elib.belstu.by/handle/123456789/14460 2013-01-01T00:00:00Z https://elib.belstu.by/handle/123456789/14461 Title: Vitamins and plant saponina in production of functional margarines and spreads possessing antioxidant properties Authors: Medvedev, D. A. Abstract: The composition of margarine products and spreads with the increased emulsion stability and prolonged expiration date is developed in the factory laboratory. A distinctive feature is the use of plant saponins as emulsifiers and ascorbic acid as an antioxidant water-soluble component. The use of these additional ingredients makes margarines and spreads the products of functional food. It is established that the use of saponins and vitamins with antioxidant properties increases the expiration date of the product. It was shown that the licorice saponins and ascorbic acid inhibit hydrolysis and lipid peroxidation (the speed of growth of acid number and concentration of malondialdehyde). Tue, 01 Jan 2013 00:00:00 GMT https://elib.belstu.by/handle/123456789/14461 2013-01-01T00:00:00Z