Complex analysis of flax seeds composition for breeding programs

Abstract

Single plant selection using complex of seed quality traits requires development and optimization of the methods, which allow qualitative and quantitative analysis applying minimum quantity of seeds. The proposed evaluation scheme of seed composition enables optimal use of seed material for individual plant analysis: oil percentage (Rushkovsky’s extraction method), fatty acid composition (vaporliquid chromatography), protein and ash content (dynamic thermogravimetry), elemental composition (electron probe X-ray fluorescence method). Reproducibility of analytical methods used was assessed by the coefficient of variation, the reproducibility index (ARI) and the convergence of data on seasonal cultivation.