Development of method for determination of beta-agonists content in meat and dairy products by enzyme immunoassay

Abstract

The article presents the results of the development of methods for determining the content of beta-agonists in livestock products. The paper analyzes the issue of the use of hormones in the feed for farm animals, existing threats and risks to human health, determines the maximum allowable levels of clenbuterol residues and ractopamine in the animal products, describes the existing methods for determining the content of beta-agonists physical-chemical and chemical-biological methods. The objects of investigation were frozen beef slabs, beef liver, milk. The content of ractopamine and clenbuterol in the samples was determined by ELISA method. Based on the data basic the metrological characteristics of the determination of clenbuterol in animal products were established – the detectable concentration range from 0.04 to 2000 ng/kg, the relative standard deviation of repeatability 0.8% (meat), 0.7% (milk), the relative standard deviation of intermediate precision of 1.0% (meat), 0.9% (milk), and ractopamine – detectable concentration range from 0.02 to 8,100 ng/kg, the relative standard deviation of repeatability 0.3% (meat), 0.4% (beef liv er), the relative standard deviation of the interme-diate precision of 0.8% (meat) and 0.9% (beef liver ). Extended standard uncertainty measurements of clenbuterol and ractopamine did not exceed 1%. The results will be used to develop the methods for determining the content of beta -agonists by ELISA quality control and safety of animal products for human consumption.