Development of molecular genetic approaches to predict the occurance of foci of bacterial diseases in forest ecosystems

Abstract

In the course of the study, specific primers were developed to identify 20 species of phytopathogenic bacteria from 12 genera associated with the occurrence of infectious diseases in seven forest-forming species of Belarus. The results showed that the developed oligonucleotides are specific for the 16S (mtDNA and cpDNA) and 23S rRNA genes of the studied phytopathogenic bacteria. At a further stage of research, in vitro tests will be carried out to establish the effectiveness of diagnosing bacterial marker loci, both using pure cultures of pathogen isolates and samples of affected tissues of woody plants.