Influence of macro- and micro-element content on mycelial growth of Phoma sp.1

Abstract

This report provides additional data about the influence of macro- and micro-elements on the growth of Phoma sp. 1 in vitro to complement the description of growth culture and morphological features. It was found that addition of tricalcium phosphate and potassium chloride to the media at concentrations of 1 g/l and 2 g/l slows the mycelial growth by an average of 30%. The micro-elements copper sulphate (at 50 mg/l and 100 mg/l) and zinc sulphate (at 100 mg/l) decreased growth by 50%, 63%, and 32%, respectively. Urea completely inhibited Ph. sp.1 growth at 0.5 g/l. Summarising previously published information, Phoma sp. 1 is a mesophilic acidophile, capable of forming chlamydospores in culture. Pycnidia form deep in the media only after prolonged low-temperature storage. The optimal pH for growth is 4.3–4.9. Constant medium aeration favours the formation of typical mycelium, proportional growth and stable biomass accumulation.